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Product Name
DEK antibody
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Description
DEK Mouse Monoclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, Jurkat cells. Positive IHC detected in human brain tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
D6S231E antibody; DEK antibody; DEK oncogene antibody; Protein DEK antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of DEK recombinant protein (Accession Number: XM_024446544). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107238(DEK Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:107238(DEK Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:107238(DEK Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG
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Background
Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptinoal regulaton and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.
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References
- Yi HC, Liu YL, You P. Overexpression of DEK gene is correlated with poor prognosis in hepatocellular carcinoma. Molecular medicine reports. 11(2):1318-23. 2015.
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