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Product Name
DEFA1 antibody
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Description
DEFA1 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human small intestine tissue, human liver cancer tissue. Observed molecular weight by Western-blot: 6 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
DEF1 antibody; DEFA1 antibody; DEFA1B antibody; DEFA2 antibody; defensin antibody; alpha 1 antibody; HNP 1 antibody; HNP 2 antibody; HP 1 antibody; HP 2 antibody; HP1 antibody; HP2 antibody; MRS antibody; Neutrophil defensin 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DEFA1 recombinant protein (Accession Number: NM_004084). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109849(DEFA1 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:109849(DEFA1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:109849(DEFA1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:109849(DEFA1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DEFA1 represents defensin, alpha 1
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"