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Product Name
DDX39 antibody
- Documents
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Description
DDX39 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, A431 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse testis tissue, Raji cells. Positive IHC detected in human testis tissue, human kidney tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 49-55 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BAT1 antibody; BAT1L antibody; DDX39 antibody; DDX39A antibody; DDXL antibody; DEAD box protein 39 antibody; Nuclear RNA helicase URH49 antibody; URH49 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DDX39 recombinant protein (Accession Number: BC032128). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109828(DDX39 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:109828(DDX39 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:109828(DDX39 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using DDX39 antibody Catalog No:109828 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-DDX39 (IP:Catalog No:109828, 4ug; Detection:Catalog No:109828 1:1000) with HEK-293 cells lysate 3000ug.
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Background
DDX39 represents DEAD (Asp-Glu-Ala-Asp) box polypeptide 39
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"