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Product Name
DCTN6 antibody
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Description
DCTN6 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human kidney tissue, human spleen tissue. Positive WB detected in mouse lung tissue, mouse testis tissue, rat testis tissue. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 27kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DCTN6 antibody; dynactin 6 antibody; Dynactin subunit 6 antibody; Dynactin subunit p27 antibody; Protein WS 3 antibody; WS 3 antibody; WS3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DCTN6 recombinant protein (Accession Number: NM_006571). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:109761(DCTN6 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:109761(DCTN6 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:109761(DCTN6 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-DCTN6 (IP:Catalog No:109761, 4ug; Detection:Catalog No:109761 1:500) with mouse testis tissue lysate 4800ug.
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Background
DCTN6 represents dynactin 6
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References
- Yeh TY, Kowalska AK, Scipioni BR. Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit. The EMBO journal. 32(7):1023-35. 2013.
- Cavolo SL, Zhou C, Ketcham SA. Mycalolide B dissociates dynactin and abolishes retrograde axonal transport of dense-core vesicles. Molecular biology of the cell. 26(14):2664-72. 2015.
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