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Product Name
DAXX antibody
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Description
DAXX Rabbit Polyclonal antibody. Positive IF detected in A431 cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 120 kDa,70 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
BING2 antibody; DAP6 antibody; DAXX antibody; EAP1 antibody; ETS1 assOCiated protein 1 antibody; hDaxx antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DAXX recombinant protein (Accession Number: NM_001350). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109871(DAXX antibody) at dilution of 1:1000
Immunofluorescent analysis of A431 cells using Catalog No:109871(DAXX Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DAXX represents death-domain associated protein
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References
- Begum NA, Stanlie A, Nakata M, Akiyama H, Honjo T. The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation. The Journal of biological chemistry. 287(39):32415-29. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"