Product Name

DACH1 antibody

Documents

Description

DACH1 Rabbit Polyclonal antibody. Positive IF detected in HEK-293 cells. Positive IHC detected in human prostate cancer tissue. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HEK-293. Observed molecular weight by Western-blot: 97-100 kDa

Tested applications

ELISA, IHC, IP, WB, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

DACH antibody; DACH1 antibody; Dachshund homolog 1 antibody; Dachshund-1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of DACH1 recombinant protein (Accession Number: BC021219). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IP: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

HEK293 cells (Native, siRNA-control, siRNA-DACH1) were subjected to SDS PAGE followed by western blot with Catalog No:109853 (DACH1 antibody) at dilution of 1:1000

IP Result of anti-DACH1 (IP:Catalog No:109853, 4ug; Detection:Catalog No:109853 1:1000) with HEK-293 cells lysate 6000ug.

Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109853(DACH1 antibody) at dilution of 1:50 (under 10x lens)

HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109853(DACH1 Antibody) at dilution of 1:600

Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using Catalog No:109853(DACH1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1.

References

Wu L, Herman JG, Brock MV. Silencing DACH1 promotes esophageal cancer growth by inhibiting TGF-β signaling. PloS one. 9(4):e95509. 2014.
Yan W, Wu K, Herman JG. Epigenetic silencing of DACH1 induces the invasion and metastasis of gastric cancer by activating TGF-β signalling. Journal of cellular and molecular medicine. 18(12):2499-511. 2014.
Dincer Z, Piao J, Niu L. Specification of functional cranial placode derivatives from human pluripotent stem cells. Cell reports. 5(5):1387-402. 2013.
Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L. Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage. Genes & development. 22(2):152-65. 2008.
Ladewig J, Koch P, Brüstle O. Auto-attraction of neural precursors and their neuronal progeny impairs neuronal migration. Nature neuroscience. 17(1):24-6. 2014.
Chu Q, Han N, Yuan X. DACH1 inhibits cyclin D1 expression, cellular proliferation and tumor growth of renal cancer cells. Journal of hematology & oncology. 7:73. 2014.
Chen HI, Sharma B, Akerberg BN. The sinus venosus contributes to coronary vasculature through VEGFC-stimulated angiogenesis. Development (Cambridge, England). 141(23):4500-12. 2014.
Mendes LO, Scarano WR, Rochel-Maia SS. Androgen therapy reverses injuries caused by ethanol consumption in the prostate: testosterone as a possible target to ethanol-related disorders. Life sciences. 120:22-30. 2015.

DACH1 antibody

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