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Product Name
CYTSA antibody
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Description
CYTSA Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in NIH/3T3 cells, HeLa cells, mouse brain tissue, Neuro-2a cells. Positive IF detected in HepG2 cells. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 130 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
cytospin A antibody; CYTSA antibody; KIAA0376 antibody; SPECC1 like protein antibody; SPECC1L antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CYTSA recombinant protein (Accession Number: NM_001145468). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:109815(CYTSA Antibody) at dilution of 1:600
IP Result of anti-CYTSA (IP:Catalog No:109815, 4ug; Detection:Catalog No:109815 1:1000) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:109815(CYTSA Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:109815(CYTSA Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:109815(CYTSA Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CYTSA, also named as SPECC1L or KIAA0376, is a 1117 amino acid protein, which contains one CH domain and belongs to the cytospin-A family. CYTSA localizes in the cytoplasm and is involved in cytokinesis and spindle organization. CYTSA may play a role in actin cytoskeleton organization and microtubule stabilization, hence it is required for proper cell adhesion and migration.
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