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Product Name
Cytokeratin 8 antibody
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Description
Cytokeratin 8 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, A431 cells, A549 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human lung cancer tissue, human breast cancer tissue, human colon cancer tissue, human colon tissue, human liver tissue, human oesophagus cancer tissue, human prostate cancer tissue, human stomach tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 54 kDa
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Tested applications
ELISA, WB, IF, FC, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CARD2 antibody; CK 8 antibody; CK8 antibody; CYK8 antibody; Cytokeratin 8 antibody; K2C8 antibody; keratin 8 antibody; KRT8 antibody; Type II keratin Kb8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002273). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:109814(KRT8 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:109814(KRT8 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:109814(KRT8 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:109814(KRT8 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HeLa cells were stained with 0.2ug KRT8 antibody (Catalog No:109814, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.
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References
- Vijayalingam S, Kuppuswamy M, Subramanian T. Evaluation of apoptogenic adenovirus type 5 oncolytic vectors in a Syrian hamster head and neck cancer model. Cancer gene therapy. 21(6):228-37. 2014.
- Hou R, Wang YW, Liang HF. Animal and cellular models of hepatocellular carcinoma bone metastasis: establishment and characterisation. Journal of cancer research and clinical oncology. 141(11):1931-43. 2015.
- Wei S, Zou Q, Lai S. Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators. Scientific reports. 6:19648. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"