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Product Name
Cytokeratin 7-specific antibody
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Description
Cytokeratin 7-specific Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue, human breast cancer tissue, human cervical cancer tissue, human liver cancer tissue, human liver tissue, human lung cancer tissue. Positive IF detected in HepG2 cells, Hela cells. Positive IP detected in HepG2 cells. Positive WB detected in A431 cells, HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 54 kDa
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Tested applications
ELISA, WB, IF, IP, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CK 7 antibody; CK7 antibody; Cytokeratin 7 antibody; K2C7 antibody; keratin 7 antibody; KRT7 antibody; Sarcolectin antibody; SCL antibody; Type II keratin Kb7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005556). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:109811(CK7-Specific Antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:109811(KRT7-Specific antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:109811(KRT7-Specific antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0
Immunofluorescent analysis of HepG2 cells using Catalog No:109811(CK7-Specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-CK7-Specific (IP:Catalog No:109811, 4ug; Detection:Catalog No:109811 1:1000) with HepG2 cells lysate 3600ug.
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. This antibody is specifically against KRT7.
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References
- Zhang QJ, He J, Ni W. Noninvasive urine-derived cell lines derived from neurological genetic patients. Neuroreport. 24(4):161-6. 2013.
- Noguchi A, Kikuchi K, Ohtsu T. Pulmonary tumors associated with the JC virus T-antigen in a transgenic mouse model. Oncology reports. 30(6):2603-8. 2013.
- Wang J, Liu S, Qin HM, Zhao Y, Wang XQ, Yan Q. Pregnancy-associated plasma protein A up-regulated by progesterone promotes adhesion and proliferation of trophoblastic cells. International journal of clinical and experimental pathology. 7(4):1427-37. 2014.
- Wen J, Xiao Y, Wang J. Low doses of CMV induce autoimmune-mediated and inflammatory responses in bile duct epithelia of regulatory T cell-depleted neonatal mice. Laboratory investigation; a journal of technical methods and pathology. 95(2):180-92. 2015.
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