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Product Name
Cytokeratin 17-Specific antibody
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Description
Cytokeratin 17-Specific Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue, human lung cancer tissue. Positive IF detected in HeLa cells. Positive FC detected in HeLa cells. Positive WB detected in A431 cells, HEK-293 cells, HeLa cells, mouse skin tissue. Positive IP detected in A431 cells. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, IHC, FC, IP, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
39.1 antibody; CK 17 antibody; Cytokeratin 17 antibody; K17 antibody; keratin 17 antibody; KRT17 antibody; PC2 antibody; PCHC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_000422). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:109798(KRT17-Specific Antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:109798(KRT17-Specific antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:109798(KRT17-Specific antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:109798(KRT17-Specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-KRT17-Specific (IP:Catalog No:109798, 4ug; Detection:Catalog No:109798 1:1000) with A431 cells lysate 1200ug.
1X10^6 HeLa cells were stained with 0.2ug KRT17-Specific antibody (Catalog No:109798, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.
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