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Product Name
Cytokeratin 1-specific antibody
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Description
Cytokeratin 1-specific Rabbit Polyclonal antibody. Positive IHC detected in human skin tissue, human cervix tissue, human lung cancer tissue. Positive IF detected in A431 cells. Positive IP detected in A431 cells. Positive WB detected in mouse skin tissue, A375 cells, A431 cells. Observed molecular weight by Western-blot: 67kd
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Tested applications
ELISA, IP, IF, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
67 kDa cytokeratin antibody; CK 1 antibody; CK1 antibody; Cytokeratin 1 antibody; keratin 1 antibody; KRT1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_006121). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human skin using Catalog No:109804(KRT1-specific antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin using Catalog No:109804(KRT1-specific antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-KRT1-specific (IP:Catalog No:109804, 3ug; Detection:Catalog No:109804 1:500) with A431 cells lysate 3500ug.
mouse skin tissue were subjected to SDS PAGE followed by western blot with Catalog No:109804(KRT1-specific Antibody) at dilution of 1:1000
Immunofluorescent analysis of A431 cells using Catalog No:109804(KRT1-specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. This antibody is specifically against ketatin I.
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