-
Product Name
Cytohesin 2 antibody
- Documents
-
Description
Cytohesin 2 Rabbit Polyclonal antibody. Positive WB detected in mouse heart tissue. Positive IP detected in mouse heart tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 47 kDa
-
Tested applications
ELISA, IHC, IF, WB, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
ARF exchange factor antibody; ARNO antibody; CTS18 antibody; CTS18.1 antibody; CYTH2 antibody; cytohesin 2 antibody; Protein ARNO antibody; PSCD2 antibody; PSCD2L antibody; SEC7L antibody; Sec7p L antibody; Sec7p like antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Cytohesin 2 recombinant protein (Accession Number: NM_017457). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:109788(CYTH2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:109788(CYTH2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using CYTH2 antibody Catalog No:109788 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CYTH2 (IP:Catalog No:109788, 4ug; Detection:Catalog No:109788 1:500) with mouse heart tissue lysate 4000ug.
-
Background
Pleckstrin homology, Sec7 and coiled/coil domains 2 (PSCD2) is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. PSCD2 exhibits GEP activity in vitro with small G proteins ARF1, ARF3, and ARF6.
-
References
- Xu K, Gao J, Yang X, Yao Y, Liu Q. Cytohesin-2 as a novel prognostic marker for hepatocellular carcinoma. Oncology reports. 29(6):2211-8. 2013.
- Zhai J, Zhang L, Mojsilovic-Petrovic J. Inhibition of Cytohesins Protects against Genetic Models of Motor Neuron Disease. The Journal of neuroscience : the official journal of the Society for Neuroscience. 35(24):9088-105. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"