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Product Name
Cytochrome c antibody
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Description
Cytochrome c Mouse Monoclonal antibody. Positive IHC detected in human breast cancer tissue, human liver cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in human skeletal muscle tissue, human heart tissue. Observed molecular weight by Western-blot: 12-15 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
CYC antibody; CYCS antibody; CytOChrome c antibody; cytOChrome c antibody; somatic antibody; HCS antibody; THC4 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of Cyt℃hrome c recombinant protein (Accession Number: NM_018947). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:5000-1:50000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:107227(Cytochrome c Antibody) at dilution of 1:16000
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (Cytochrome c Antibody) at dilution of 1:600 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (Cytochrome c Antibody) at dilution of 1:600 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:107227(Cytochrome c Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.
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