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Product Name
CYTB antibody
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Description
CYTB Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in MCF-7 cells. Observed molecular weight by Western-blot: 43 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
COB antibody; Complex III subunit 3 antibody; Complex III subunit III antibody; CYTB antibody; cytOChrome b antibody; MT CYB antibody; MTCYB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: YP_003024038). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IF: 1:20-1:200
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Validations
MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:109782(CYTB Antibody) at dilution of 1:300
Immunofluorescent analysis of HeLa cells using Catalog No:109782(CYTB Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CYTB, also named as COB and MTCYB, is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. It’s mitochondrion membrane protein with MW 43 kDa.
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References
- Jiang P, Jin X, Peng Y. The exome sequencing identified the mutation in YARS2 encoding the mitochondrial tyrosyl-tRNA synthetase as a nuclear modifier for the phenotypic manifestation of Leber's hereditary optic neuropathy-associated mitochondrial DNA mutation. Human molecular genetics. 25(3):584-96. 2016.
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