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Product Name
CYP2J2 antibody
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Description
CYP2J2 Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue, human heart tissue. Positive WB detected in mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 58kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Arachidonic acid epoxygenase antibody; CPJ2 antibody; CYP2J2 antibody; CYPIIJ2 antibody; CytOChrome P450 2J2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CYP2J2 recombinant protein (Accession Number: NM_000775). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:109696(CYP2J2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109696(CYP2J2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109696(CYP2J2 antibody) at dilution of 1:50 (under 40x lens)
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Background
CYP2J2(Cytochrome P450 2J2) belongs to the cytochrome P450 family. This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. The human CYP2J member, CYP2J2, is shown to be expressed in several tissues such as the small intestine, liver, heart, kidney, and lung and may play a role in the oxidative bioactivation of arachidonic acid(PMID:12386130).
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