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Product Name
CYP2E1-Specific antibody
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Description
CYP2E1-Specific Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive WB detected in Jurkat cells, mouse liver tissue. Observed molecular weight by Western-blot: 50-57kd,38kd
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Tested applications
ELISA, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
4 nitrophenol 2 hydroxylase antibody; CPE1 antibody; CYP2E antibody; CYP2E1 antibody; CYP-2E1 antibody; CYPIIE1 antibody; CytOChrome P450 2E1 antibody; CytOChrome P450 J antibody; P450 J antibody; P450C2E antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_000773). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
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Validations
Immunofluorescent analysis of Hela cells, using CYP2E1 antibody Catalog No:109694 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109694(CYP2E1-Specific Antibody) at dilution of 1:1000
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Background
CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. The antibody is specific to CYP2E1.
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References
- Jiang W, Gao M, Sun S. Protective effect of L-theanine on carbon tetrachloride-induced acute liver injury in mice. Biochemical and biophysical research communications. 422(2):344-50. 2012.
- Zhao X, Cong X, Zheng L, Xu L, Yin L, Peng J. Dioscin, a natural steroid saponin, shows remarkable protective effect against acetaminophen-induced liver damage in vitro and in vivo. Toxicology letters. 214(1):69-80. 2012.
- Song J, Zhao M, Liu X, Zhu Y, Hu X, Chen F. Protection of cyanidin-3-glucoside against oxidative stress induced by acrylamide in human MDA-MB-231 cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 58:306-10. 2013.
- Zhang S, Zheng L, Dong D. Effects of flavonoids from Rosa laevigata Michx fruit against high-fat diet-induced non-alcoholic fatty liver disease in rats. Food chemistry. 141(3):2108-16. 2013.
- Dong D, Zhang S, Yin L. Protective effects of the total saponins from Rosa laevigata Michx fruit against carbon tetrachloride-induced acute liver injury in mice. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 62:120-30. 2013.
- Xu T, Zheng L, Xu L. Protective effects of dioscin against alcohol-induced liver injury. Archives of toxicology. 88(3):739-53. 2014.
- Lee JK, Chung HJ, Fischer L, Fischer J, Gonzalez FJ, Jeong H. Human placental lactogen induces CYP2E1 expression via PI 3-kinase pathway in female human hepatocytes. Drug metabolism and disposition: the biological fate of chemicals. 42(4):492-9. 2014.
- Yu H, Zheng L, Yin L. Protective effects of the total saponins from Dioscorea nipponica Makino against carbon tetrachloride-induced liver injury in mice through suppression of apoptosis and inflammation. International immunopharmacology. 19(2):233-44. 2014.
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