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Cyclophilin A antibody

Cat.#: 109673

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Product Information

  • Product Name
    Cyclophilin A antibody
  • Documents
  • Description
    Cyclophilin A Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IF detected in Hela cells. Positive IHC detected in human colon cancer tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue, human tonsillitis tissue. Positive WB detected in K-562 cells, C6 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, Raji cells. Observed molecular weight by Western-blot: 18kd
  • Tested applications
    ELISA, WB, IHC, IF, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Cyclophilin A antibody; Cyclosporin A binding protein antibody; CYPA antibody; CYPH antibody; PPIA antibody; PPIase A antibody; Rotamase A antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Cyclophilin A recombinant protein (Accession Number: NM_021130). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109673(CYPA antibody) at dilution of 1:1500

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109673(CYPA antibody) at dilution of 1:1500

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109673(CYPA antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109673(CYPA antibody) at dilution of 1:200 (under 10x lens)

    Immunofluorescent analysis of HepG2 cells, using PPIA antibody Catalog No:109673 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using PPIA antibody Catalog No:109673 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    1X10^6 HeLa cells were stained with 0.2ug CYPA antibody (Catalog No:109673, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug CYPA antibody (Catalog No:109673, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.
  • References
    • Tian X, Zhao C, Zhu H. Hepatitis B virus (HBV) surface antigen interacts with and promotes cyclophilin a secretion: possible link to pathogenesis of HBV infection. Journal of virology. 84(7):3373-81. 2010.
    • Watanabe A, Yoneda M, Ikeda F, Terao-Muto Y, Sato H, Kai C. CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells. Journal of virology. 84(9):4183-93. 2010.
    • Huang CF, Sun ZJ, Zhao YF, Chen XM, Jia J, Zhang WF. Increased expression of peroxiredoxin 6 and cyclophilin A in squamous cell carcinoma of the tongue. Oral diseases. 17(3):328-34. 2011.
    • Huang C, Sun Z, Sun Y. Association of increased ligand cyclophilin A and receptor CD147 with hypoxia, angiogenesis, metastasis and prognosis of tongue squamous cell carcinoma. Histopathology. 60(5):793-803. 2012.
    • He H, Mou Z, Li W. Proteomic methods reveal cyclophilin A function as a host restriction factor against rotavirus infection. Proteomics. 13(7):1121-32. 2013.
    • Nishioku T, Dohgu S, Koga M. Cyclophilin A secreted from fibroblast-like synoviocytes is involved in the induction of CD147 expression in macrophages of mice with collagen-induced arthritis. Journal of inflammation (London, England). 9(1):44. 2012.
    • Huang CF, Zhang L, Ma SR. Clinical significance of Keap1 and Nrf2 in oral squamous cell carcinoma. PloS one. 8(12):e83479. 2013.
    • Wu P, Zhu X, Jin W, Hao S, Liu Q, Zhang L. Oxaliplatin triggers necrosis as well as apoptosis in gastric cancer SGC-7901 cells. Biochemical and biophysical research communications. 460(2):183-90. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"