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Product Name
Cyclin B2 antibody
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Description
Cyclin B2 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human cervical cancer tissue, human lung cancer tissue, human testis tissue. Positive IP detected in A431 cells. Positive WB detected in A431 cells, K-562 cells. Observed molecular weight by Western-blot: 51 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CCNB2 antibody; cyclin B2 antibody; G2/mitotic specific cyclin B2 antibody; HsT17299 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Cyclin B2 recombinant protein (Accession Number: NM_004701). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:109663(CCNB2 antibody) at dilution of 1:500
Immunofluorescent analysis of Hela cells, using CCNB2 antibody Catalog No:109663 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109663(CCNB2 Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109663(CCNB2 Antibody) at dilution of 1:200 (under 40x lens)
IP Result of anti-CCNB2 (IP:Catalog No:109663, 4ug; Detection:Catalog No:109663 1:1000) with A431 cells lysate 2000ug.
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Background
CCNB2 represents cyclin B2
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