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Cyclin A2 antibody

Cat.#: 109661

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Product Information

  • Product Name
    Cyclin A2 antibody
  • Documents
  • Description
    Cyclin A2 Rabbit Polyclonal antibody. Positive WB detected in HL-60 cells, K-562 cells, MCF7 cells, MCF-7 cells, NIH/3T3 cells, U-937 cells. Positive IP detected in HL-60 cells. Positive IF detected in HeLa cells, MCF-7 cells. Observed molecular weight by Western-blot: 56 kDa
  • Tested applications
    ELISA, IP, WB, IF
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CCN1 antibody; CCNA antibody; CCNA2 antibody; Cyclin A antibody; cyclin A2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Cyclin A2 recombinant protein (Accession Number: NM_001237). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IF: 1:50-1:500

  • Validations

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109661(Cyclin A2 Antibody) at dilution of 1:600

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109661(Cyclin A2 Antibody) at dilution of 1:600

    IP Result of anti-Cyclin A2 (IP:Catalog No:109661, 3ug; Detection:Catalog No:109661 1:500) with HL-60 cells lysate 4000ug.

    IP Result of anti-Cyclin A2 (IP:Catalog No:109661, 3ug; Detection:Catalog No:109661 1:500) with HL-60 cells lysate 4000ug.

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:109661(Cyclin A2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:109661(Cyclin A2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. CCNA2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
  • References
    • Hsu TI, Wang MC, Chen SY. Betulinic acid decreases specificity protein 1 (Sp1) level via increasing the sumoylation of sp1 to inhibit lung cancer growth. Molecular pharmacology. 82(6):1115-28. 2012.
    • Wang W, Wang HJ, Wang B. The Role of the Y Box Binding Protein 1 C-Terminal Domain in Vascular Endothelial Cell Proliferation, Apoptosis, and Angiogenesis. DNA and cell biology. 35(1):24-32. 2016.
    • Song X, Li L, Shi Q. Polychlorinated Biphenyl Quinone Metabolite Promotes p53-Dependent DNA Damage Checkpoint Activation, S-Phase Cycle Arrest and Extrinsic Apoptosis in Human Liver Hepatocellular Carcinoma HepG2 Cells. Chemical research in toxicology. 28(11):2160-9. 2015.
    • Ye WL, Zhao YP, Li HQ. Doxorubicin-poly (ethylene glycol)-alendronate self-assembled micelles for targeted therapy of bone metastatic cancer. Scientific reports. 5:14614. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"