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Product Name
CYB5B antibody
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Description
CYB5B Rabbit Polyclonal antibody. Positive IP detected in mouse lung tissue. Positive WB detected in mouse lung tissue, HepG2 cells, human adrenal gland tissue, human liver tissue. Positive IHC detected in human testis tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 20-21 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CYB5 M antibody; CYB5B antibody; CYB5M antibody; CYPB5M antibody; CytOChrome b5 type B antibody; DKFZp686M0619 antibody; OMB5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CYB5B recombinant protein (Accession Number: BC004373). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:109655(CYB5B antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:109655(CYB5B antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:109655(CYB5B antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-CYB5B (IP:Catalog No:109655, 3ug; Detection:Catalog No:109655 1:500) with mouse lung tissue lysate 4000ug.
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Background
CYB5B(Cytochrome b5 type B) is also named as CYB5M, OMB5 and belongs to the cytochrome b5 family. It is a 21 kDa membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. This protein is expressed on the plasma membrane of lymphoma cells but not normal lymphocytes, reactive lymphocytes or bone marrow precursor cells(PMID:20100355). The full length protein has a propeptide with 11 amino acids.
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