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Product Name
CXCR7 antibody
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Description
CXCR7 Rabbit Polyclonal antibody. Positive FC detected in Raji cells. Positive IHC detected in human kidney tissue, human brain tissue, human liver tissue, human lung tissue, human pancreas cancer tissue, human placenta tissue, human spleen tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive WB detected in C6 cells, A549 cells. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, IHC, IF, WB, FC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
Chemokine orphan receptor 1 antibody; CMKOR1 antibody; CXC R7 antibody; CXCR 7 antibody; CXCR7 antibody; G protein coupled receptor 159 antibody; GPR159 antibody; RDC 1 antibody; RDC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: XM_047445135). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
C6 cells were subjected to SDS PAGE followed by western blot with Catalog No:109646(CXCR7 antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109646(CXCR7 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109646(CXCR7 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CXCR7 antibody Catalog No:109646 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 Raji cells were stained with 0.2ug CXCR7 antibody (Catalog No:109646, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
CXCR7, also named as CMKOR1, GPR159 and RDC1, belongs to the G-protein coupled receptor 1 family. It is a receptor for CXCL12/SDF1. CXCR7 acts as coreceptor with CXCR4 for a restricted number of HIV isolates. CXCR7 was shown to contribute to neoangiogenesis in prostate cancer and to promote tumor cell proliferation in breast cancer and adenocarcinoma of the lung.
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References
- Baumhoer D, Smida J, Zillmer S. Strong expression of CXCL12 is associated with a favorable outcome in osteosarcoma. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 25(4):522-8. 2012.
- Yu J, Wang A, Tang Z. The effect of stromal cell-derived factor-1α/heparin coating of biodegradable vascular grafts on the recruitment of both endothelial and smooth muscle progenitor cells for accelerated regeneration. Biomaterials. 33(32):8062-74. 2012.
- Brennecke P, Arlt MJ, Muff R. Expression of the chemokine receptor CXCR7 in CXCR4-expressing human 143B osteosarcoma cells enhances lung metastasis of intratibial xenografts in SCID mice. PloS one. 8(9):e74045. 2013.
- Liu Z, Yang L, Teng X, Zhang H, Guan H. The involvement of CXCR7 in modulating the progression of papillary thyroid carcinoma. The Journal of surgical research. 191(2):379-88. 2014.
- Bi J, Li P, Li C. The SDF-1/CXCR4 chemokine axis in uveal melanoma cell proliferation and migration. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2015.
- Kremer D, Cui QL, Göttle P. CXCR7 Is Involved in Human Oligodendroglial Precursor Cell Maturation. PloS one. 11(1):e0146503. 2016.
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