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Product Name
CUL4A antibody
- Documents
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Description
CUL4A Mouse Monoclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human heart tissue, human breast cancer tissue. Positive IP detected in MCF-7 cells. Positive WB detected in Hela cells. Observed molecular weight by Western-blot: 77 kDa; 88 kDa
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Tested applications
ELISA, WB, IP, IF, IHC
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Species reactivity
Human, Monkey, Mouse, Rat; other species not tested.
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Alternative names
CUL 4A antibody; CUL4A antibody; cullin 4A antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of CUL4A recombinant protein (Accession Number: NM_001008895). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107182(CUL4A antibody) at dilution of 1:500
IP Result of anti-CUL4A (IP:Catalog No:107182, 4ug; Detection:Catalog No:107182 1:500) with MCF-7 cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:107182(CUL4A Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:107182(CUL4A Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of HepG2 cells using Catalog No:107182(CUL4A Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2. This antibody is specially against CUL4A, it will not cross-react with CUL4B.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"