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Product Name
CUL1 antibody
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Description
CUL1 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue. Positive WB detected in BxPC-3 cells, K-562 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 90 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
CUL 1 antibody; CUL1 antibody; cullin 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CUL1 recombinant protein (Accession Number: BC034318). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
BxPC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:109741(CUL1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:109741(CUL1 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-CUL1 (IP:Catalog No:109741, 3ug; Detection:Catalog No:109741 1:300) with HeLa cells lysate 2000ug.
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Background
The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1–3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"