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Product Name
CUGBP2 antibody
- Documents
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Description
CUGBP2 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, mouse thymus tissue. Positive IP detected in mouse skeletal muscle tissue. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat,Zebrafish; other species not tested.
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Alternative names
Bruno like protein 3 antibody; BRUNOL3 antibody; CELF 2 antibody; CELF2 antibody; CUG BP2 antibody; CUGBP2 antibody; ETR 3 antibody; ETR3 antibody; hNAPOR antibody; NAPOR antibody; RNA binding protein BRUNOL 3 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CUGBP2 recombinant protein (Accession Number: BC036391). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109740(CUGBP2 antibody) at dilution of 1:300
IP Result of anti-CUGBP2 (IP:Catalog No:109740, 3ug; Detection:Catalog No:109740 1:300) with mouse skeletal muscle tissue lysate 7500ug.
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109740(CUGBP2 antibody) at dilution of 1:100 (under 10x lens)
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Background
The CELF (CUGBP and ETR-3 Like Factor)/Bruno-like protein family plays important roles in the regulation of alternative splicing and translation. CUGBP2 is a member of this family of proteins that possesses several alternatively spliced exons. CUGBP2 induces smooth muscle-specific exon inclusion via binding to uridine purine repeat elements (URE) in ACTN1. Although CUGBP2 is known as a splicing activator for the N-methyl-D-aspartate receptor 1 (NMDA R1) exon 21, CUGBP2 represses NMDA R1 exon 5 inclusion . In addition, CUGBP2 was reported to repress the inclusion of the Irexon 11
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References
- Otsuka N, Tsuritani K, Sakurai T. Transcriptional induction and translational inhibition of Arc and Cugbp2 in mice hippocampus after transient global ischemia under normothermic condition. Brain research. 1287:136-45. 2009.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"