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Product Name
CUGBP1 antibody
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Description
CUGBP1 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human skin cancer tissue, human testis tissue. Positive IF detected in Hela cells. Positive WB detected in HeLa cells, HL-60 cells, HT-1080 cells, NIH/3T3 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 51-55kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Bruno like protein 2 antibody; BRUNOL2 antibody; CELF 1 antibody; CELF1 antibody; CUG BP antibody; CUG BP1 antibody; CUGBP antibody; CUGBP1 antibody; Deadenylation factor CUG BP antibody; EDEN BP homolog antibody; hNab50 antibody; NAB50 antibody; RNA binding protein BRUNOL 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CUGBP1 recombinant protein (Accession Number: XM_011519859). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109739(CUGBP1 antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109739(CUGBP1 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using CUGBP1 antibody Catalog No:109739 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-CUGBP1 (IP:Catalog No:109739, 4ug; Detection:Catalog No:109739 1:500) with HeLa cells lysate 3200ug.
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Background
CUGBP1 represents CUG triplet repeat, RNA binding protein 1
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References
- Galloway JN, Shaw C, Yu P. CGG repeats in RNA modulate expression of TDP-43 in mouse and fly models of fragile X tremor ataxia syndrome. Human molecular genetics. 23(22):5906-15. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"