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Product Name
CTNNA3 antibody
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Description
CTNNA3 Rabbit Polyclonal antibody. Positive WB detected in mouse testis tissue. Positive IP detected in mouse testis tissue. Positive IHC detected in human liver cancer tissue, human heart tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 100kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Alpha T catenin antibody; Cadherin assOCiated protein antibody; Catenin alpha 3 antibody; CTNNA3 antibody; VR22 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CTNNA3 recombinant protein (Accession Number: BC030646). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:109725(CTNNA3 antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109725(CTNNA3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109725(CTNNA3 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CTNNA3 antibody Catalog No:109725 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-CTNNA3 (IP:Catalog No:109725, 5ug; Detection:Catalog No:109725 1:1500) with mouse testis tissue lysate 4000ug.
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Background
CTNNA3 represents catenin (cadherin-associated protein), alpha 3
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References
- Fanjul-Fernández M, Quesada V, Cabanillas R. Cell-cell adhesion genes CTNNA2 and CTNNA3 are tumour suppressors frequently mutated in laryngeal carcinomas. Nature communications. 4:2531. 2013.
- Jäger S, Cimermancic P, Gulbahce N. Global landscape of HIV-human protein complexes. Nature. 481(7381):365-70. 2012.
- Li J, Gao E, Vite A. Alpha-catenins control cardiomyocyte proliferation by regulating Yap activity. Circulation research. 116(1):70-9. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"