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Product Name
CTBS antibody
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Description
CTBS Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, HeLa cells, HepG2 cells, mouse colon tissue, SMMC-7721 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human prostate cancer tissue, human kidney tissue, human liver cancer tissue, human liver tissue. Observed molecular weight by Western-blot: 44-50kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
chitobiase antibody; di N acetyl antibody; CTB antibody; CTBS antibody; Di N acetylchitobiase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CTBS recombinant protein (Accession Number: BC024007). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:109718(CTBS antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109718(CTBS antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CTBS antibody Catalog No:109718 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
CTBS(Di-N-acetylchitobiase (chitobiase)) also named as CTB, is a lysosomal exoglycosidase that splits the GlcNAcβ-D-(l-4)GlcNAc chitobiose core of asparagine-linked glycoproteins. Chitobiase has been purified from human liver and kidney and rat liver. Both the human and rat enzymes are approximately 40-45 kDa and require a free reducing end GlcNAc for activity(PMID:1527079). This full length protein has a signal peptide and four glycosylation sites.
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