-
Product Name
CSTF1 antibody
- Documents
-
Description
CSTF1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, K-562 cells. Positive IP detected in K-562 cells. Observed molecular weight by Western-blot: 50kd
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human; other species not tested.
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of CSTF1 recombinant protein (Accession Number: NM_001033521). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109617(CSTF1 antibody) at dilution of 1:500
IP Result of anti-CSTF1 (IP:Catalog No:109617, 4ug; Detection:Catalog No:109617 1:500) with K-562 cells lysate 1540ug.
-
Background
Cleavage stimulation factor (CstF) , a heterotrimeric protein with subunits of 77, 64, and 50 kD (CstF-77, -64, and -50), is a polyadenylation factor that helps to specify the site of processing. CstF recognizes the G+U-rich element, a sequence located downstream of the cleavage site. CstF-50 unit interacts with the COOH-terminal domain of the RNAP II largest subunit (CTD).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"