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Product Name
CSDE1 antibody
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Description
CSDE1 Rabbit Polyclonal antibody. Positive IHC detected in human skin cancer tissue, human testis tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells, HEK-293 cells. Observed molecular weight by Western-blot: 89kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CSDE1 antibody; D1S155E antibody; DKFZp779B0247 antibody; DKFZp779J1455 antibody; FLJ26882 antibody; KIAA0885 antibody; N ras upstream gene protein antibody; NRU antibody; Protein UNR antibody; RP5 1000E10.3 antibody; UNR antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CSDE1 recombinant protein (Accession Number: NM_001242893). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109592(UNR; CSDE1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:109592(UNR; CSDE1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:109592(UNR; CSDE1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:109592(UNR; CSDE1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CSDE1 (Cold shock domain-containing protein E1), also known as UNR (upstream of N-ras), is an RNA-binding protein involved in the regulation of messenger RNA stability and internal initiation of translation. It is required for internal initiation of translation of human rhinovirus RNA. UNR may be involved in translationally coupled mRNA turnover. Reduced expression of UNR impairs proliferation and differentiation of erythroid blasts. Recent study found that UNR acts as a positive or negative regulator of cell death, depending on the cell type, suggesteing manipulation of UNR level may constitute a specific approach to sensitize cancer cells to anticancer treatments.
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References
- Horos R, Ijspeert H, Pospisilova D. Ribosomal deficiencies in Diamond-Blackfan anemia impair translation of transcripts essential for differentiation of murine and human erythroblasts. Blood. 119(1):262-72. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"