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Product Name
CROT antibody
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Description
CROT Rabbit Polyclonal antibody. Positive WB detected in mouse kidney tissue. Positive IHC detected in human liver cancer tissue. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 65-70kd
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
COT antibody; CROT antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CROT recombinant protein (Accession Number: NM_021151). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:109577(CROT antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109577(CROT antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109577(CROT antibody) at dilution of 1:100 (under 40x lens)
1X10^6 HepG2 cells were stained with 0.2ug CROT antibody (Catalog No:109577, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
CROT, also named as COT, belongs to the carnitine/choline acetyltransferase family. It is a beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. CROT converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CROT is an active forms for carnitine octanoyltransferase. This antibody can bind the close sequences genes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"