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Product Name
CRMP4 antibody
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Description
CRMP4 Rabbit Polyclonal antibody. Positive IP detected in SH-SY5Y cells. Positive WB detected in SH-SY5Y cells, Jurkat cells, mouse cerebellum tissue, mouse thymus tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue. Observed molecular weight by Western-blot: 60-70 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CRMP 4 antibody; CRMP4 antibody; dihydropyrimidinase like 3 antibody; DPYSL3 antibody; DRP 3 antibody; DRP3 antibody; LCRMP antibody; ULIP antibody; ULIP 1 antibody; ULIP1 antibody; Unc 33 like phosphoprotein 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CRMP4 recombinant protein (Accession Number: NM_001387). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:109573(DPYSL3 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109573(DPYSL3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109573(DPYSL3 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using DPYSL3 antibody Catalog No:109573 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-DPYSL3 (IP:Catalog No:109573, 3ug; Detection:Catalog No:109573 1:700) with SH-SY5Y cells lysate 3000ug.
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Background
DPYSL3(Dihydropyrimidinase-related protein 3) is also named as CRMP4, DRP3, ULIP, ULIP1 and belongs to the hydantoinase/dihydropyrimidinase subfamily.DPYSL3 is a developmentally regulated protein, strongly expressed in early embryonic post-mitotic neural cells, reaching a peak of expression around the first post-natal week. In the adult brain, DPYSL3 is present mainly in regions that retain neurogenesis.DPYSL3 protein is also found in synaptic sites at the adult neuromuscular junction where it may be involved in the maintenance of neuronal stability(PMID: 16135096).It has 2 isoforms produced by alternative splicing.
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References
- Tokuda K, Kuramitsu Y, Byron B. Up-regulation of DRP-3 long isoform during the induction of neural progenitor cells by glutamate treatment in the ex vivo rat retina. Biochemical and biophysical research communications. 463(4):593-9. 2015.
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