Creatine kinase B type-Specific antibody

Cat.#: 109544

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Product Information

  • Product Name
    Creatine kinase B type-Specific antibody
  • Documents
  • Description
    Creatine kinase B type-Specific Rabbit Polyclonal antibody. Positive IHC detected in human gliomas. Positive WB detected in HEK-293 cells, mouse colon tissue, Y79 cells. Observed molecular weight by Western-blot: 43kd
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    B CK antibody; CKB antibody; CKBB antibody; Creatine kinase B chain antibody; Creatine kinase B type antibody; creatine kinase antibody; brain antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_001823). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IHC: 1:20-1:200

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109544(CKB-Specific antibody) at dilution of 1:1000

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109544(CKB-Specific antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109544(CKB-Specific antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109544(CKB-Specific antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109544(CKB-Specific antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109544(CKB-Specific antibody) at dilution of 1:100 (under 40x lens)

  • Background
    CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"