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Product Name
CPT1C-specific antibody
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Description
CPT1C-specific Rabbit Polyclonal antibody. Positive IP detected in mouse testis tissue. Positive WB detected in mouse brain tissue. Positive IHC detected in human testis tissue, human brain tissue, human gliomas tissue. Observed molecular weight by Western-blot: 70-82 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CATL1 antibody; CPT IC antibody; CPT1 B antibody; CPT1C antibody; CPT1P antibody; CPTI B antibody; CPTIC antibody; FLJ23809 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CPT1C-specific recombinant protein (Accession Number: XM_047438159). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109532(CPT1C antibody) at dilution of 1:500
IP Result of anti-CPT1C (IP:Catalog No:109532, 5ug; Detection:Catalog No:109532 1:500) with mouse testis tissue lysate 8000ug.
Immunohistochemical of paraffin-embedded human testis using Catalog No:109532(CPT1C antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:109532(CPT1C antibody) at dilution of 1:50 (under 40x lens)
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Background
CPT1C, also named as CATL1, CPTI-B and B-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1C is an active forms of related brain-type carnitine palmitoyltransferase I. CPT1C may be a regulated target of malonyl-CoA that relays the “malonyl-CoA signal” in hypothalamic neurons that express the orexigenic and anorexigenic neuropeptides that regulate food intake and peripheral energy expenditure. (PMID: 17018521 ) This antibody specifically recognizes CPT1C, and does not cross-react with CPT1A or CPT1B.
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References
- Lage R, Vázquez MJ, Varela L. Ghrelin effects on neuropeptides in the rat hypothalamus depend on fatty acid metabolism actions on BSX but not on gender. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 24(8):2670-9. 2010.
- Sangiao-Alvarellos S, Varela L, Vázquez MJ. Influence of ghrelin and growth hormone deficiency on AMP-activated protein kinase and hypothalamic lipid metabolism. Journal of neuroendocrinology. 22(6):543-56. 2010.
- Gao S, Zhu G, Gao X. Important roles of brain-specific carnitine palmitoyltransferase and ceramide metabolism in leptin hypothalamic control of feeding. Proceedings of the National Academy of Sciences of the United States of America. 108(23):9691-6. 2011.
- Huang D, Li T, Li X. HIF-1-mediated suppression of acyl-CoA dehydrogenases and fatty acid oxidation is critical for cancer progression. Cell reports. 8(6):1930-42. 2014.
- Martínez de Morentin PB, Lage R, González-García I. Pregnancy induces resistance to the anorectic effect of hypothalamic malonyl-CoA and the thermogenic effect of hypothalamic AMPK inhibition in female rats. Endocrinology. 156(3):947-60. 2015.
- Rinaldi C, Schmidt T, Situ AJ. Mutation in CPT1C Associated With Pure Autosomal Dominant Spastic Paraplegia. JAMA neurology. 72(5):561-70. 2015.
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