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Product Name
CPSF4 antibody
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Description
CPSF4 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HeLa cells, HepG2 cells. Positive IP detected in HeLa cells. Positive IHC detected in human endometrial cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CPSF 30 kDa subunit antibody; CPSF30 antibody; CPSF4 antibody; NAR antibody; Neb 1 antibody; NEB1 antibody; No arches homolog antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CPSF4 recombinant protein (Accession Number: NM_001081559). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109527(CPSF4 antibody) at dilution of 1:300
IP Result of anti-CPSF4 (IP:Catalog No:109527, 3ug; Detection:Catalog No:109527 1:400) with HeLa cells lysate 1000ug.
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:109527(CPSF4 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CPSF4 antibody Catalog No:109527 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
The mature 3-prime ends of cellular mRNAs are producted by endonucleolytic cleavage of the primary transcripts, followed by polyadenylation of the upstream cleavage products. The cleavage and polyadenylation specificity factor (CPSF) has a essential role in pre-mRNA 3-prime end processing in that it is required in both the cleavage step and subsequent poly(A) addition [PMID: 9651582]. CPSF4 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex, whose other componet is CPSF1, CPSF2, CPSF3 and FIP1L1.
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References
- Chen W, Guo W, Li M. Upregulation of cleavage and polyadenylation specific factor 4 in lung adenocarcinoma and its critical role for cancer cell survival and proliferation. PloS one. 8(12):e82728. 2013.
- Chen W, Qin L, Wang S. CPSF4 activates telomerase reverse transcriptase and predicts poor prognosis in human lung adenocarcinomas. Molecular oncology. 8(3):704-16. 2014.
- Tang Z, Yu W, Zhang C. CREB-binding protein regulates lung cancer growth by targeting MAPK and CPSF4 signaling pathway. Molecular oncology. 10(2):317-29. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"