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Product Name
CPLX2 antibody
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Description
CPLX2 Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue. Positive IP detected in SH-SY5Y cells. Positive WB detected in human brain tissue, mouse brain tissue. Observed molecular weight by Western-blot: 18-20 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
921 L antibody; complexin 2 antibody; Complexin II antibody; CPLX2 antibody; CPX 2 antibody; CPX II antibody; CPX2 antibody; Hfb1 antibody; Synaphin 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CPLX2 recombinant protein (Accession Number: XM_005265799). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109511(CPLX2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:109511(CPLX2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:109511(CPLX2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-CPLX2 (IP:Catalog No:109511, 3ug; Detection:Catalog No:109511 1:700) with SH-SY5Y cells lysate 800ug.
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Background
Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.
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References
- Wang Z, Wei X, Liu K. NOX2 deficiency ameliorates cerebral injury through reduction of complexin II-mediated glutamate excitotoxicity in experimental stroke. Free radical biology & medicine. 65:942-51. 2013.
- Walus M, Kida E, Rabe A, Albertini G, Golabek AA. Widespread cerebellar transcriptome changes in Ts65Dn Down syndrome mouse model after lifelong running. Behavioural brain research. 296:35-46. 2016.
- Komatsu H, Kakehashi A, Nishiyama N. Complexin-2 (CPLX2) as a potential prognostic biomarker in human lung high grade neuroendocrine tumors. Cancer biomarkers : section A of Disease markers. 13(3):171-80. 2013.
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