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Product Name
CP110 antibody
- Documents
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Description
CP110 Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IF detected in MEF/HeLa cells. Positive IHC detected in human prostate cancer tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, K-562 cells. Observed molecular weight by Western-blot: 113kd
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Tested applications
ELISA, WB, IHC, IF, IP, FC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
Centrosomal protein of 110 kDa antibody; CP110 antibody; CP110 protein antibody; DKFZp781G1416 antibody; KIAA0419 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CP110 recombinant protein (Accession Number: NM_001323572). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
IP result of CP110(Catalog No:109504) by Dr. Corbit, Kevin.
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109504(CP110 antibody) at dilution of 1:100 (under 10x lens)
IF result(Centrioles) of anti-CP110(Catalog No:109504) by Dr. Corbit Kevin.
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109504(CP110 antibody) at dilution of 1:400
IP Result of anti-CP110 (IP:Catalog No:109504, 3ug; Detection:Catalog No:109504 1:1000) with HeLa cells lysate 3000ug.
1X10^6 HeLa cells were stained with .2ug CP110 antibody (Catalog No:109504, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.
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References
- Cao J, Shen Y, Zhu L. miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics. Nature cell biology. 14(7):697-706. 2012.
- Kodani A, Salomé Sirerol-Piquer M, Seol A, Garcia-Verdugo JM, Reiter JF. Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages. The EMBO journal. 32(4):597-607. 2013.
- Li J, D'Angiolella V, Seeley ES. USP33 regulates centrosome biogenesis via deubiquitination of the centriolar protein CP110. Nature. 495(7440):255-9. 2013.
- Joo K, Kim CG, Lee MS. CCDC41 is required for ciliary vesicle docking to the mother centriole. Proceedings of the National Academy of Sciences of the United States of America. 110(15):5987-92. 2013.
- Wang WJ, Tay HG, Soni R. CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base. Nature cell biology. 15(6):591-601. 2013.
- Zhao H, Zhu L, Zhu Y. The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis. Nature cell biology. 15(12):1434-44. 2013.
- Xu Q, Zhang Y, Xiong X. PIPKIγ targets to the centrosome and restrains centriole duplication. Journal of cell science. 127(Pt 6):1293-305. 2014.
- Ye X, Zeng H, Ning G, Reiter JF, Liu A. C2cd3 is critical for centriolar distal appendage assembly and ciliary vesicle docking in mammals. Proceedings of the National Academy of Sciences of the United States of America. 111(6):2164-9. 2014.
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