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Product Name
COX5B antibody
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Description
COX5B Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, HeLa cells, human heart tissue, human liver tissue, rat liver tissue, L02 cells. Positive IHC detected in human gliomas. Positive IF detected in MCF-7. Observed molecular weight by Western-blot: 14 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
COX5B antibody; COXVB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of COX5B recombinant protein (Accession Number: NM_001862). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109492(COX5B antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109492(COX5B antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using COX5B antibody Catalog No:109492 at 1:50 antibody and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)
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References
- Oliva CR, Nozell SE, Diers A. Acquisition of temozolomide chemoresistance in gliomas leads to remodeling of mitochondrial electron transport chain. The Journal of biological chemistry. 285(51):39759-67. 2010.
- Broadwater L, Pandit A, Clements R. Analysis of the mitochondrial proteome in multiple sclerosis cortex. Biochimica et biophysica acta. 1812(5):630-41. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"