Product Name

COX2 antibody

Documents

Description

COX2 Rabbit Polyclonal antibody. Positive WB detected in LPS treated HEK-293 cells, HEK-293 cells, LPS treated RAW264.7 cells, mouse embryo tissue, rat stomach tissue, RAW264.7 cells. Positive IHC detected in human ovary tissue, human colon cancer tissue, human kidney tissue, human lung cancer tissue, human lung tissue, human placenta tissue, human prostate hyperplasia tissue, human testis tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 70-74 kDa, 64 kDa

Tested applications

ELISA, WB, IHC, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

COX 2 antibody; COX2 antibody; Cyclooxygenase 2 antibody; GRIPGHS antibody; hCox 2 antibody; PGG/HS antibody; PGH synthase 2 antibody; PGHS 2 antibody; PHS 2 antibody; PHS II antibody; Prostaglandin G/H synthase 2 antibody; Prostaglandin H2 synthase 2 antibody; PTGS2 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of COX2 recombinant protein (Accession Number: NM_000963). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

LPS treated HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109488(PTGS2 antibody) at dilution of 1:600

Immunohistochemical of paraffin-embedded human ovary using Catalog No:109488(PTGS2 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human ovary using Catalog No:109488(PTGS2 antibody) at dilution of 1:50 (under 40x lens)

Immunofluorescent analysis of HepG2 cells using Catalog No:109488(COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Background

COX2 (Prostaglandin G/H synthase 2; PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72–74 kDa and the aglycosylated is 66 kDa(PMID:19656660 ). It also expresses a band of 39 kDa after unspecific cleavage(PMID:17509125 ). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains(PMID:14724276 ).

References

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Ding QH, Cheng Y, Chen WP, Zhong HM, Wang XH. Celastrol, an inhibitor of heat shock protein 90β potently suppresses the expression of matrix metalloproteinases, inducible nitric oxide synthase and cyclooxygenase-2 in primary human osteoarthritic chondrocytes. European journal of pharmacology. 708(1-3):1-7. 2013.
Zhang S, Qi Y, Xu Y. Protective effect of flavonoid-rich extract from Rosa laevigata Michx on cerebral ischemia-reperfusion injury through suppression of apoptosis and inflammation. Neurochemistry international. 63(5):522-32. 2013.
Dong D, Zhang S, Yin L. Protective effects of the total saponins from Rosa laevigata Michx fruit against carbon tetrachloride-induced acute liver injury in mice. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 62:120-30. 2013.
Liu YW, Zhu X, Zhang L. Cerebroprotective effects of ibuprofen on diabetic encephalopathy in rats. Pharmacology, biochemistry, and behavior. 117:128-36. 2014.
Tian F, Zhang YJ, Li Y, Xie Y. Celecoxib ameliorates non-alcoholic steatohepatitis in type 2 diabetic rats via suppression of the non-canonical Wnt signaling pathway expression. PloS one. 9(1):e83819. 2014.
Yu H, Zheng L, Yin L. Protective effects of the total saponins from Dioscorea nipponica Makino against carbon tetrachloride-induced liver injury in mice through suppression of apoptosis and inflammation. International immunopharmacology. 19(2):233-44. 2014.
Wang J, Yu L, Jiang C. Cerebral ischemia increases bone marrow CD4+CD25+FoxP3+ regulatory T cells in mice via signals from sympathetic nervous system. Brain, behavior, and immunity. 43:172-83. 2015.

COX2 antibody

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