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Product Name
COTL1 antibody
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Description
COTL1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human lung cancer tissue, human kidney tissue. Positive IP detected in mouse kidney tissue. Positive WB detected in mouse brain tissue, HEK-293 cells, HeLa cells, human brain tissue, human kidney tissue, human placenta tissue, mouse placenta tissue, rat brain tissue. Observed molecular weight by Western-blot: 16 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
CLP antibody; Coactosin like protein antibody; COTL1 antibody; FLJ43657 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of COTL1 recombinant protein (Accession Number: NM_021149). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109480(COTL1 antibody) at dilution of 1:1000
IP Result of anti-COTL1 (IP:Catalog No:109480, 3ug; Detection:Catalog No:109480 1:3000) with mouse kidney tissue lysate 12500ug.
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:109480(COTL1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:109480(COTL1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using COTL1 antibody Catalog No:109480 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.
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References
- Somalinga BR, Day CE, Wei S, Roth MG, Thomas PJ. TDP-43 identified from a genome wide RNAi screen for SOD1 regulators. PloS one. 7(4):e35818. 2012.
- Sun W, Guo C, Meng X. Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential. Journal of investigative medicine : the official publication of the American Federation for Clinical Research. 60(4):689-94. 2012.
- Schröder M, Häfner AK, Hofmann B. Stabilisation and characterisation of the isolated regulatory domain of human 5-lipoxygenase. Biochimica et biophysica acta. 1842(10):1538-47. 2014.
- Jeong HC, Kim GI, Cho SH. Proteomic analysis of human small cell lung cancer tissues: up-regulation of coactosin-like protein-1. Journal of proteome research. 10(1):269-76. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"