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Product Name
Cortactin antibody
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Description
Cortactin Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, MCF7 cells, NIH/3T3 cells. Positive IP detected in HEK-293 cells. Positive FC detected in HeLa cells. Positive IF detected in HeLa cells, HepG2 cells. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 75~80 kDa
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Tested applications
ELISA, WB, IHC, FC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Amplaxin antibody; cortactin antibody; CTTN antibody; EMS1 antibody; Oncogene EMS1 antibody; Src substrate cortactin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Cortactin recombinant protein (Accession Number: NM_138565). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109478(CTTN antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:109478(CTTN antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-Cortactin (IP:Catalog No:109478, 4ug; Detection:Catalog No:109478 1:300) with HEK-293 cells lysate 2000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:109478(Cortactin Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HeLa cells were stained with 0.2ug Cortactin antibody (Catalog No:109478, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phennotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it migrates as multiple bands between 75 and 85 kDa in SDS-PAGE.
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