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Product Name
COPS3 antibody
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Description
COPS3 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human kidney tissue, human heart tissue, human liver tissue, human lung tissue, human placenta tissue, human spleen tissue, human testis tissue. Positive FC detected in MCF-7 cells. Positive WB detected in human brain tissue, mouse uterus tissue. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
COPS3 antibody; CSN3 antibody; SGN3 antibody; Signalosome subunit 3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of COPS3 recombinant protein (Accession Number: NM_003653). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109463(COPS3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109463(COPS3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109463(COPS3 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using COPS3 antibody Catalog No:109463 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 MCF-7 cells were stained with 0.2ug COPS3 antibody (Catalog No:109463, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
COPS3 represents COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"