Product Name

COP1 antibody

Documents

Description

COP1 Rabbit Polyclonal antibody. Positive IHC detected in human osteosarcoma tissue. Positive WB detected in human heart tissue. Observed molecular weight by Western-blot: 90kd

Tested applications

ELISA, WB, IHC

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

COP1 antibody; FLJ10416 antibody; hCOP1 antibody; RFWD2 antibody; RING finger protein 200 antibody; RNF200 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of COP1 recombinant protein (Accession Number: BC039723). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

Validations

human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:109456(COP1 antibody) at dilution of 1:500

Immunohistochemical of paraffin-embedded human osteosarcoma using Catalog No:109456(COP1 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human osteosarcoma using Catalog No:109456(COP1 antibody) at dilution of 1:100 (under 40x lens)

Background

COP1 is also names as RFWD2,RNF200 and belongs to the COP1 family. It is a RING E3 ubiquitin ligase that targets key regulators for degradation, and DET1 complexes with COP10 and DDB1, which is proposed to aid in COP1-mediated degradation (PMID:22705257). The endogenous COP1 is localized predominantly in the nucleus, but small amount may also be present in the cytosol. Within the nucleus, COP1 is present in both the nucleoplasm (NP) and the nuclear envelope (NE) fractions, although COP1 is more enriched in the nucleoplasm(PMID:12466024). It has 4 isoforms produced by alternative splicing. This antibody can recognize the 4 isoform of COP1.

COP1 antibody

Product Information

Related Products / Services