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Product Name
COMT antibody
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Description
COMT Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, A431 cells, BxPC-3 cells. Positive IP detected in K-562 cells. Positive FC detected in HepG2 cells. Positive IHC detected in human liver tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, IHC, IF, FC, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
catechol O methyltransferase antibody; COMT antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of COMT recombinant protein (Accession Number: BC000419). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human liver using Catalog No:109448(COMT antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:109448(COMT antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using COMT antibody Catalog No:109448 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109448(COMT Antibody) at dilution of 1:600
IP Result of anti-COMT (IP:Catalog No:109448, 4ug; Detection:Catalog No:109448 1:500) with K-562 cells lysate 1200ug.
1X10^6 HepG2 cells were stained with 0.2ug COMT antibody (Catalog No:109448, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.
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References
- Saller S, Merz-Lange J, Raffael S. Norepinephrine, active norepinephrine transporter, and norepinephrine-metabolism are involved in the generation of reactive oxygen species in human ovarian granulosa cells. Endocrinology. 153(3):1472-83. 2012.
- Prunotto M, Farina A, Lane L. Proteomic analysis of podocyte exosome-enriched fraction from normal human urine. Journal of proteomics. 82:193-229. 2013.
- Liu Z, Luo X, Liu L. Histone acetyltransferase p300 promotes MKL1-mediated transactivation of catechol-O-methyltransferase gene. Acta biochimica et biophysica Sinica. 45(12):1002-10. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"