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Product Name
Collagen Type VIII antibody
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Description
Collagen Type VIII Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, A375 cells, COLO 320 cells, HeLa cells, mouse spleen tissue. Observed molecular weight by Western-blot: 73 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C3orf7 antibody; COL8A1 antibody; Collagen alpha 1(VIII) chain antibody; Collagen Type VIII antibody; collagen antibody; type VIII antibody; alpha 1 antibody; Endothelial collagen antibody; Vastatin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Collagen Type VIII recombinant protein (Accession Number: NM_020351). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109440(COL8A1 antibody) at dilution of 1:500
IP Result of anti-COL8A1 (IP:Catalog No:109440, 4ug; Detection:Catalog No:109440 1:500) with HepG2 cells lysate 1800ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:109440(COL8A1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
COL8A1 represents collagen, type VIII, alpha 1
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