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COLEC11 antibody

Cat.#: 109374

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Product Information

  • Product Name
    COLEC11 antibody
  • Documents
  • Description
    COLEC11 Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, human blood tissue, rat liver tissue. Positive IP detected in mouse liver tissue. Positive IF detected in ATCD5 cells. Observed molecular weight by Western-blot: 34 kDa
  • Tested applications
    ELISA, WB, IF, IP
  • Species reactivity
    Human,Mouse,Rat,Zebrafish; other species not tested.
  • Alternative names
    CL K1 antibody; CL K1 I antibody; CL K1 II antibody; CL K1 IIa antibody; CL K1 IIb antibody; CLK1 antibody; COLEC11 antibody; Collectin 11 antibody; Collectin kidney protein 1 antibody; collectin sub family member 11 antibody; DKFZp686N1868 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of COLEC11 recombinant protein (Accession Number: NM_024027). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IF: 1:20-1:200

  • Validations

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109374(COLEC11 antibody) at dilution of 1:500

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109374(COLEC11 antibody) at dilution of 1:500

    IF result(confocal image) of CL-K1 in ATCD5 cell show Golgi localization from Prof. Philip L. Beales.

    IF result(confocal image) of CL-K1 in ATCD5 cell show Golgi localization from Prof. Philip L. Beales.

    IP Result of anti-COLEC11 (IP:Catalog No:109374, 4ug; Detection:Catalog No:109374 1:500) with mouse liver tissue lysate 6400ug.

    IP Result of anti-COLEC11 (IP:Catalog No:109374, 4ug; Detection:Catalog No:109374 1:500) with mouse liver tissue lysate 6400ug.

  • Background
    COLEC11, also named as CL-K1 and Collectin-11, belongs to the COLEC10/COLEC11 family. It is a lectin that binds to various sugars: fucose > mannose. It does not bind to glucose, N-acetylglucosamine and N-acetylgalactosamine. COLEC11 binds to LPS. COLEC11 and MASP1 are two genes in the lectin complement pathway, they are mutated in 3MC syndrome, implicating this diverse inflammation-chemotaxis cascade in the etiology of human developmental disorders. COLEC11 serves as a guidance cue for neural crest cell migration.(PMID:21258343)
  • References
    • Rooryck C, Diaz-Font A, Osborn DP. Mutations in lectin complement pathway genes COLEC11 and MASP1 cause 3MC syndrome. Nature genetics. 43(3):197-203. 2011.
    • Ma YJ, Skjoedt MO, Garred P. Collectin-11/MASP complex formation triggers activation of the lectin complement pathway--the fifth lectin pathway initiation complex. Journal of innate immunity. 5(3):242-50. 2013.
    • Rosbjerg A, Munthe-Fog L, Garred P, Skjoedt MO. Heterocomplex formation between MBL/ficolin/CL-11-associated serine protease-1 and -3 and MBL/ficolin/CL-11-associated protein-1. Journal of immunology (Baltimore, Md. : 1950). 192(9):4352-60. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"