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Product Name
Coilin antibody
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Description
Coilin Rabbit Polyclonal antibody. Positive FC detected in HEK-293T cells. Positive IF detected in HepG2 cells. Positive IHC detected in human prostate cancer tissue, human testis tissue. Positive IP detected in HEK-293 cells. Positive WB detected in human brain tissue, HEK-293 cells, Jurkat cells, K-562 cells. Observed molecular weight by Western-blot: 80kd
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Tested applications
ELISA, WB, IHC, FC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CLN80 antibody; COIL antibody; Coilin antibody; p80 antibody; p80 coilin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Coilin recombinant protein (Accession Number: NM_004645). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109372(COIL antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109372(COIL antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-COIL (IP:Catalog No:109372, 3ug; Detection:Catalog No:109372 1:500) with HEK-293 cells lysate 1000ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:109372(COIL Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HEK-293T cells were stained with 0.2ug COIL antibody (Catalog No:109372, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs
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References
- Chen S, Evans HG, Evans DR. FLASH knockdown sensitizes cells to Fas-mediated apoptosis via down-regulation of the anti-apoptotic proteins, MCL-1 and Cflip short. PloS one. 7(3):e32971. 2012.
- Gu Z, Li Y, Lee P, Liu T, Wan C, Wang Z. Protein arginine methyltransferase 5 functions in opposite ways in the cytoplasm and nucleus of prostate cancer cells. PloS one. 7(8):e44033. 2012.
- Dury AY, El Fatimy R, Tremblay S. Nuclear Fragile X Mental Retardation Protein is localized to Cajal bodies. PLoS genetics. 9(10):e1003890. 2013.
- Pawellek A, McElroy S, Samatov T. Identification of small molecule inhibitors of pre-mRNA splicing. The Journal of biological chemistry. 289(50):34683-98. 2014.
- Mertens-Walker I, Lisle JE, Nyberg WA. EphB4 localises to the nucleus of prostate cancer cells. Experimental cell research. 333(1):105-15. 2015.
- Pawallek A, McElroy S, Samatov T. Identification of small molecule inhibitors of pre-mRNA splicing. The Journal of biological chemistry. 290(10):6005. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"