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Product Name
CNN3 antibody
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Description
CNN3 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue, human hysteromyoma tissue, human skin tissue. Positive IF detected in Hela cells. Positive IP detected in HEK-293 cells. Positive WB detected in human kidney tissue, human skeletal muscle tissue. Observed molecular weight by Western-blot: 36 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Calponin 3 antibody; calponin 3 antibody; acidic antibody; Calponin antibody; acidic isoform antibody; CNN3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CNN3 recombinant protein (Accession Number: NM_001839). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:109428(CNN3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109428(CNN3 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using CNN3 antibody Catalog No:109428 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-CNN3 (IP:Catalog No:109428, 4ug; Detection:Catalog No:109428 1:500) with HEK-293 cells lysate 3000ug.
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Background
CNN3 represents calponin 3, acidic
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