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Product Name
CNN2 antibody
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Description
CNN2 Mouse Monoclonal antibody. Positive WB detected in pig stomach tissue, HT-1080 cells. Positive IHC detected in human breast hyperplasia tissue. Observed molecular weight by Western-blot: 34-36 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human, Pig; other species not tested.
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Alternative names
calponin 2 antibody; Calponin H2 antibody; smooth muscle antibody; CNN2 antibody; Neutral calponin antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of CNN2 recombinant protein (Accession Number: NM_004368). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:500-1:4000
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Validations
pig stomach tissue were subjected to SDS PAGE followed by western blot with Catalog No:107166(CNN2 Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human breast hyperplasia tissue slide using Catalog No:107166(CNN2 Antibody) at dilution of 1:2000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human breast hyperplasia tissue slide using Catalog No:107166(CNN2 Antibody) at dilution of 1:2000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain.
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