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Product Name
CLTCL1 antibody
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Description
CLTCL1 Mouse Monoclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, HEK-293 cells, Jurkat cells. Observed molecular weight by Western-blot: 180 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
CHC22 antibody; Clathrin heavy chain 2 antibody; clathrin antibody; heavy chain like 1 antibody; CLH 22 antibody; CLH22 antibody; CLTCL antibody; CLTCL1 antibody; CLTD antibody; FLJ36032 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_007098). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with (CLTCL1 Antibody) at dilution of 1:1000
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:107164(CLTCL1 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
CLTCL1, also named as CLH22, CLTCL and CLTD, belongs to the clathrin heavy chain family. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. The antibody is specific to CLTCL1. It has no cross reaction to CLTC.
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