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Product Name
CLPP antibody
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Description
CLPP Rabbit Polyclonal antibody. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in K-562 cells, human placenta tissue, rat heart tissue. Positive IHC detected in human heart tissue, human brain tissue, human kidney tissue, human liver cancer tissue, human liver tissue, human lung tissue, human ovary tissue, human pancreas tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 26 kDa; 30 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CLPP antibody; Endopeptidase Clp antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CLPP recombinant protein (Accession Number: NM_006012). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109401(CLPP antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human heart using Catalog No:109401(CLPP antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:109401(CLPP antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using CLPP antibody Catalog No:109401 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CLPP (IP:Catalog No:109401, 3ug; Detection:Catalog No:109401 1:500) with mouse skeletal muscle tissue lysate 4000ug.
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Background
CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.
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References
- Moore F, Santin I, Nogueira TC. The transcription factor C/EBP delta has anti-apoptotic and anti-inflammatory roles in pancreatic beta cells. PloS one. 7(2):e31062. 2012.
- Gispert S, Parganlija D, Klinkenberg M. Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors. Human molecular genetics. 22(24):4871-87. 2013.
- Mishra P, Carelli V, Manfredi G, Chan DC. Proteolytic cleavage of Opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation. Cell metabolism. 19(4):630-41. 2014.
- Khan NA, Auranen M, Paetau I. Effective treatment of mitochondrial myopathy by nicotinamide riboside, a vitamin B3. EMBO molecular medicine. 6(6):721-31. 2014.
- Richter U, Lahtinen T, Marttinen P, Suomi F, Battersby BJ. Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness. The Journal of cell biology. 211(2):373-89. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"